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GE17-0510-10

Q Sepharose Fast Flow

Cytiva 17-0510-10, pack of 25 mL

Synonym(e):

Q Sepharose Fast Flow

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About This Item

UNSPSC-Code:
41106500
NACRES:
NA.56

ligand

quaternary amine

Beschreibung

Ion Exchanger Type (value)

Verpackung

pack of 25 mL

Hersteller/Markenname

Cytiva 17-0510-10

Matrix

6% cross-linked agarose

Partikelgröße

45-165 μm

Durchschnittlicher Durchmesser

90 μm

cleaning in place

2-14

working range

2-12

Eignung

suitable for bioprocess medium

Allgemeine Beschreibung

Q Sepharose Fast FLow is a strong anion exchanger based on the well established Sepharose Fast FLow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Anwendung

  • Well-proven strong anion exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.

Leistungsmerkmale und Vorteile

Q Sepharose Fast FLow is part of the Sepharose Fast FLow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% Agarose beads, with quaternary ammonium (Q) strong anion exchange groups. Q Sepharose Fast FLow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with Q Sepharose Fast FLow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, Q Sepharose Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Piktogramme

Flame

Signalwort

Warning

H-Sätze

Lagerklassenschlüssel

3 - Flammable liquids


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Pär Comstedt et al.
PloS one, 9(11), e113294-e113294 (2014-11-20)
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Artikel

Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with GE Healthcare media are detailed, including sample application, load volume and temperature

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Alle anzeigen

Protokolle

This page discusses various aspects of sample preparation for chromatographic purification.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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