GE17-0729-10
SP Sepharose™ Fast Flow
Cytiva 17-0729-10, pack of 25 mL
Synonym(e):
SP Sepharose Column, Sepharose Fast Flow
About This Item
Empfohlene Produkte
ligand
sulphopropyl
Beschreibung
Ion Exchanger Type (value)
Verpackung
pack of 25 mL
Hersteller/Markenname
Cytiva 17-0729-10
Matrix
6% cross-linked agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning in place
3-14
Arbeitsbereich
4-13
Kapazität
70 mg binding capacity(ribonuclease A/ml medium)
Eignung
suitable for bioprocess medium
Allgemeine Beschreibung
Anwendung
As member of the BioProcess media range, SP Sepharose™ Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Leistungsmerkmale und Vorteile
- Well-proven strong cation exchanger developed for industrial downstream processes.
- Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
- The industry standard for ion exchange chromatography during recent decades
- High chemical stability allows for well proven CIP and sanitization protocols
- The hydrophilic nature of the base matrix ensures Low levels of non-specific binding leading to Low levels of host cell-derived impurities in the elution pool.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Artikel
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
Protokolle
This page covers the use of Sepharose Fast Flow for purification of proteins.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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