GE17-1151-01
HiTrap® Sp High Performance
Cytiva 17-1151-01, pack of 5 × 1 mL
About This Item
Empfohlene Produkte
ligand
sulphopropyl
Beschreibung
Ion Exchanger Type (value)
Verpackung
pack of 5 × 1 mL
Hersteller/Markenname
Cytiva 17-1151-01
Parameter
flow rate
42 psi
Bettgröße
7 mm × 25 mm
Bettvolumen
1 mL
Säule ID
7 mm
Matrix
6% cross-linked agarose
Partikelgröße
24-44 μm
Durchschnittliche Teilgröße
34 μm
cleaning
3-14
Arbeitsbereich
4-13
Eignung
suitable for bioprocess medium
Verwandte Kategorien
Allgemeine Beschreibung
The small particle size (34μm) of the medium allows fast adsorption and desorption even at high sample loadings and flow rates.SP Sepharose™ High Performance is a strong cation exchange medium and Q Sepharose™ High Performance is a strong anion exchange medium. Both remain charged and have high loading capacities over broad pH ranges.
Leistungsmerkmale und Vorteile
- 34 μm bead size for high-performance, high-resolution purifications.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump, and chromatography systems such as AKTA design.
- Strong sulfopropyl (SP) cation exchanger.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Ähnliches Produkt
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
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Artikel
This page shows how to perform a purification of His-tagged membrane proteins.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protokolle
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from GE Healthcare and how to analyze the refolding process with Superdex columns from GE Healthcare.
This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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