GE17-0529-01
Calmodulin-Sepharose™ 4B
Cytiva 17-0529-01, pack of 10 mL
Synonym(e):
Calmodulin Affinity Resin, Calmodulin-Sepharose
About This Item
ligand
calmodulin
Verpackung
pack of 10 mL
Hersteller/Markenname
Cytiva 17-0529-01
Lagerbedingungen
(20% Ehtanol)
Matrix
4% agarose
Partikelgröße
45-165 μm
Durchschnittlicher Durchmesser
90 μm
cleaning in place
4-9
working range
4-9
Lagertemp.
2-8°C
Allgemeine Beschreibung
Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.
Leistungsmerkmale und Vorteile
- For single-step purification of native calmodulin-binding proteins
- Suitable for tandem affinity purification (TAP) of protein complexes
- Purification of calmodulin-regulated proteins from all eukaryotic cells
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
Protokolle
This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.
Verwandter Inhalt
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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