G6008

ββ-Galactosidase aus E. coli

Grade VI, lyophilized powder, ≥250 units/mg protein

• Synonym(e): β-D-Galactosid-Galactohydrolase, Lactase
• CAS-Nummer: 9031-11-2
• EC-Nummer: 3.2.1.23 (BRENDA, IUBMB)
• EG-Nummer: 232-864-1
• MDL-Nummer: MFCD00130623
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 200
• Typ: Grade VI
• Form: lyophilized powder
• Spezifische Aktivität: ≥250 units/mg protein
• Mol-Gew.: 465 kDa
• Enthält nicht: BSA as extender
• Zusammensetzung: Protein, ≥50% biuret
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.

Anwendung

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

Biochem./physiol. Wirkung

β-Galactosidase spaltet Lactose in ihre Monosaccharid-Komponenten Glucose und Galactose. Außerdem katalysiert sie die Transglycosylierung von Glucose zu Allolactose, dem Induktor von β-Galactosidase, in einem Rückführungsmechanismus.

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physikalische Eigenschaften

Molekulargewicht des Tetramers: 465 kDa (Subunits je 116,3 kDa)

Einheitendefinition

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Physikalische Form

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)