M-1

95092201, mouse kidney, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: M-1, 95092201
• Biologische Quelle: mouse kidney
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse kidney cortical collecting duct, SV40 transformed

Beschreibung der Zelllinie

Derived from the cortical collecting duct (CCD) of a mouse, transgenic for the early region of SV40 (Tg(SV40E)Bri/7). They express many characteristics of the CCD like epithelial morphology and CCD-specific antigens. M-1 cells grown on permeable supports exhibited a high transepithelial resistance concomitant with the development of a lumen-negative transepithelial potential difference. The M-1 cell line and sub-clones exhibit principal cell (PC) functions and ß-intercalated cell (ß-ICC) functions, both typical for the heterogeneous epithelium found in the renal collecting duct. M-1 cells and sub-clones show a heterogeneous expression of PC and ß-ICC antigens with 5-10% exhibiting a dual PC/ß-ICC phenotype.

Anwendung

Kidney cell studies, differentiation studies.

Nährmedium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μm Dexamethasone (DXMT) + 5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Prolonged incubation with trypsin may be necessary for subculture.

Sonstige Hinweise

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