MES-SA/Dx-5

95051031, human uterus, Epithelial

• UNSPSC-Code: 41106514
• eCl@ss: 32190102

Eigenschaften

• Produktbezeichnung: MES-SA/Dx-5, 95051031
• Biologische Quelle: human uterus
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 47 (parent line MES-SA-45). Reported karyotype 47,XX,12qt,22q-t(5q,6p),-3,-4,-7,+t(3q?,7q),Mar1,+mar2,+Mar3
• Morphologie: Epithelial
• Produkte: High levels of Mdr1 mRNA and P-glycoprotein
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human uterus sarcoma

Beschreibung der Zelllinie

The multi drug-resistant cell line MES-SA/Dx5 was derived from the human uterine sarcoma cell line MES-SA (Sigma Catalogue number. 95051030) which was originally obtained from a tumour from a 56-year-old Caucasian female at the time of hysterectomy. The Dx5 variant exhibits a 100-fold resistance to doxorubicin and has a reported doubling time of 30 hours. The two additional marker chromosomes indicate clonal selection during drug selection. MES-SA/Dx-5 cells exhibit marked cross-resistance to a number of chemotherapeutic agents (including daunorubicin, dactinomycin, vincristine, taxol, colchicine) and moderate cross-resistance to mitomycin C and melphalan. Cross resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.

Anwendung

Tumour toxicity assays and multi-drug-resistant studies.

Nährmedium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.03% EDTA; 5% CO₂; 37°C. Tap side of flask to dislodge cells.

Sonstige Hinweise

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