COR-L23/5010

96042338, human lung, Not specified

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: COR-L23/5010, 96042338
• Biologische Quelle: human lung
• Verpackung: tube of 5 μg 96042338-DNA-5UG; pkg of vial of cells 96042338-1VL
• Wachstumsmodus: Aggregates in suspension
• Karyotyp: Not specified
• Morphologie: Not specified
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Human Caucasian lung, large cell carcinoma, drug resistant

Beschreibung der Zelllinie

COR-L23/5010 has been derived from the parent line, COR-L23 (Sigma Catalogue number. 92031919) by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). The cells overexpress multidrug resistance-associated protein (MRP) and express a 190kDa membrane-protein connected to the degree of drug-resistance. COR-L23/5010 have a higher resistance than COR-L23/R (Sigma Catalogue number. 96042339).

Anwendung

MRP studies, drug-resistance studies

DNA-Profil

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,11
D16S539: 11,13
D5S818: 10,12
D7S820: 9
THO1: 9
TPOX: 8,11
vWA: 19

Nährmedium

RPMI 1640 + 2mM Glutamine + 1.0μg/ml doxorubicin + 10% FBS

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C It is recommended to culture the cells without drug after resuscitation until the first passage.

Sonstige Hinweise

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