HT2 Clone A5E

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 92021401, mouse blood, Lymphoblast

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: mouse blood
• Verpackung: tube of 5 μg 92021401-DNA-5UG; pkg of vial of cells 92021401-1VL
• Wachstumsmodus: Suspension
• Karyotyp: Not specified
• Morphologie: Lymphoblast
• Produkte: Not specified
• Rezeptoren: Interleukin 2 (IL-2), Interleukin 4 (IL-4)
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse helper T cell

Beschreibung der Zelllinie

Derived in 1979 from BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2, the A5E clone was selected to provide a homogenous population that is highly sensitive to IL-2. This cell line expresses Thy-1 and Lyt markers in addition to high affinity IL-2 receptors. Substantial proliferation is seen in the presence of IL-4 (GM-CSF).

Anwendung

IL-2 assays

Nährmedium

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 100-200 IU/ml IL-2 + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. When starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g

Sonstige Hinweise

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