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Merck

62197

Sigma-Aldrich

Anti-Maus-IgG (Gesamtmolekül)−Atto 488 in Ziege hergestellte Antikörper

~1 mg/mL

Synonym(e):

Atto 488 Anti-Mouse IgG, Goat Anti-Mouse IgG

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About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

goat

Qualitätsniveau

Konjugat

Atto 488 conjugate

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Form

liquid

Enthält

50% glycerol as stabilizer

Speziesreaktivität

mouse

Konzentration

~1 mg/mL

Methode(n)

immunocytochemistry: 1:500
immunofluorescence: 1:400

Fluoreszenz

λex 500 nm; λem 522 nm in PBS

Versandbedingung

wet ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

Allgemeine Beschreibung

IgG is the most abundant antibody in human serum. It is secreted by B cells andis found in blood. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, that is linked together by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Anti-Mouse IgG (whole molecule)-Atto 488 antibody is specific for mouse IgG. The product is purified from goat anti-mouse IgG antiserum to remove all goat serum proteins, including immunoglobulins that do not specifically bind to mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity.

Immunogen

purified mouse IgG.

Anwendung

Anti-Mouse IgG (whole molecule)?Atto 488 antibody produced in goat has been used:
  • in immunofluorescence confocal microscopic assay for p65 localization
  • in immunohistochemical analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem./physiol. Wirkung

IgG facilitates transplacental transport and participates in the secondary immune response. It has the longest serum half life. It might be linked to allergy. IgG initiates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC).

Physikalische Form

Solution in 0.1 M sodium phosphate, 0.1 M NaCl, pH 7.5, andcontaining 5 mM sodium azide as a preservative.

Hinweis zur Analyse

frei von unkonjugiertem Farbstoff

Rechtliche Hinweise

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

IgG subclasses and allotypes: from structure to effector functions
Vidarsson G, et al.
Frontiers in Immunology, 5(1-2), 520-520 (2014)
Arnold Garbiec et al.
Arthropod structure & development, 41(2), 165-176 (2012-02-04)
Mechanisms that underlie differentiation and diversification of the ovarian follicular epithelium in insects have been best characterized in a fruit fly, Drosophila melanogaster. Recent comparative analyses have shown that dipterans evolved a common, specific system of early patterning of their
Sumeda Nandadasa et al.
PloS one, 7(6), e38756-e38756 (2012-06-22)
Alpha (α)-E-catenin is a component of the cadherin complex, and has long been thought to provide a link between cell surface cadherins and the actin skeleton. More recently, it has also been implicated in mechano-sensing, and in the control of
Emily J Reedich et al.
Journal of neuroscience research, 100(10), 1951-1966 (2022-07-16)
Spastic cerebral palsy (CP) is a movement disorder marked by hypertonia and hyperreflexia; the most prevalent comorbidity is pain. Since spinal nociceptive afferents contribute to both the sensation of painful stimuli as well as reflex circuits involved in movement, we
Carolyn L Smith et al.
The Journal of general physiology, 149(4), 483-510 (2017-03-24)
Four-domain voltage-gated Ca2+ (Cav) channels play fundamental roles in the nervous system, but little is known about when or how their unique properties and cellular roles evolved. Of the three types of metazoan Cav channels, Cav1 (L-type), Cav2 (P/Q-, N-

Artikel

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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