RAG

89040605, mouse kidney, Ameboid-like

• UNSPSC-Code: 41106514

Eigenschaften

• product name: RAG, 89040605
• Biologische Quelle: mouse kidney
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 68, hypertriploid
• Morphologie: Ameboid-like
• Produkte: ES-2
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Mouse BALB/c renal adenocarcinoma

Beschreibung der Zelllinie

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Anwendung

Hybridisation, resistance and tumourigenicity studies

Nährmedium

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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