KNRK

87061001, rat kidney, Epithelial-like

• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: KNRK, 87061001
• Biologische Quelle: rat kidney
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial-like
• Produkte: Growth factors
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Normal rat kidney, Kirsten MSV transformed

Beschreibung der Zelllinie

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

Anwendung

Transformation, growth factors and virus studies: Kirsten MSV

Nährmedium

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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