GH3

87012603, rat brain, Epithelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: GH3, 87012603
• Biologische Quelle: rat brain
• Wachstumsmodus: Adherent
• Karyotyp: Hyperdiploid, modal no. 60
• Morphologie: Epithelial
• Produkte: Growth hormone, prolactin
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Wistar-Furth pituitary tumour

Beschreibung der Zelllinie

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Anwendung

Hormone studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Sonstige Hinweise

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