LIM1899 cell line human

12062001, human colon, Epithelial; cells are small rounded and grow in clusters

• Synonym(e): LIM 1899, LIM-1899
• UNSPSC-Code: 41106514

Eigenschaften

• Produktbezeichnung: LIM1899 cell line human, 12062001
• Biologische Quelle: human colon
• Wachstumsmodus: adherent
• Morphologie: Epithelial; cells are small rounded and grow in clusters
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Beschreibung der Zelllinie

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

Nährmedium

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

Subkultur-Routine

Optimal split ratio 1:8 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.

Sonstige Hinweise

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