67309

Kovac-Reagenz für Indole

suitable for microbiology

• Synonym(e): 4-Dimethylamino-benzaldehyd -Lösung
• Empirische Formel (Hill-System): C₉H₁₁NO
• CAS-Nummer: 100-10-7
• Molekulargewicht: 149.19
• Beilstein: 4132845
• MDL-Nummer: MFCD00003381
• UNSPSC-Code: 41171621
• PubChem Substanz-ID: 329760541
• NACRES: NA.85

Eigenschaften

• Agentur: according to ISO 16654:2001
• Qualitätsniveau: 200
• Produktlinie: BioChemika
• Haltbarkeit: limited shelf life, expiry date on the label
• Zusammensetzung: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• Methode(n): microbe id | specific enzyme detection: suitable
• Anwendung(en): agriculture; clinical testing; environmental; food and beverages; microbiology
• Lagertemp.: 2-8°C
• Eignung: Escherichia coli; coliforms
• SMILES String: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• InChIKey: BGNGWHSBYQYVRX-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Anwendung

In Anwesenheit von Sauerstoff können ein paar Bakterien, z.B. E. coli, Tryptophan in Indol und alpha-Aminopropionsäure aufspalten. Dieses Reagenz dient zur Detektion von Indol und zur Identifizierung von indol-positiven und indol-negativen Mikroorganismen.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

Sicherheitshinweise

• Piktogramme: GHS02,GHS05,GHS07
• Signalwort: Danger
• H-Sätze: H226,H290,H314,H317,H332,H335
• P-Sätze: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 3
• Flammpunkt (°F): 109.4 °F
• Flammpunkt (°C): 43 °C
• Persönliche Schutzausrüstung: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter