10127655001

Glucose-6-Phosphate Dehydrogenase (G6P-DH)

grade I, from yeast

• Synonym(e): G6P-DH, Glucose-6-Phosephate Dehydrogenase
• EC-Nummer: 1.1.1.49 (BRENDA, IUBMB)
• UNSPSC-Code: 12352204

Eigenschaften

• Biologische Quelle: yeast
• Qualitätsniveau: 100
• Form: suspension
• Spezifische Aktivität: ~350 units/mg protein (At 25 °C with glucose-6-P as the substrate.)
• Verpackung: pkg of 1 mL (5 mg/ml)
• Hersteller/Markenname: Roche
• Konzentration: ≥0.1-1.0 % (w/w)
• Methode(n): activity assay: suitable
• Farbe: white to yellowish
• Optimaler pH-Wert: 9.2
• Löslichkeit: water: miscible
• Eignung: suitable for UV spectrophotometry and general use
• NCBI-Hinterlegungsnummer: CAA93357
• UniProt-Hinterlegungsnummer: P11412
• Anwendung(en): life science and biopharma
• Fremdaktivität: 6-PGDH <0.0100%; CK <0.00100%; GR <0.0100%; HK <0.0100%; PGI <0.00200%; PGluM <0.0100%
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C
• Angaben zum Gen: Saccharomyces cerevisiae ... ZWF1(855480)

Beschreibung

Allgemeine Beschreibung

Glucose-6-phosphate dehydrogenase (G6P DH) has an active dimeric form, whereas its monomeric and tetrameric forms are inactive. At low NADP⁺ and NADPH levels and under normal conditions, the enzyme exists as equilibrium of tetramers and dimers, whereas at high NADP⁺ and NADPH levels the equilibrium shifts to inactive tetramers.

Anwendung

Component of cofactor recycling systems for NADPH.

Biochem./physiol. Wirkung

Glucose-6-phosphate dehydrogenase (G6P-DH) is involved in the pentose phosphate pathway. It is responsible for catalysing the oxidation of glucose-6-phosphate (G6P) to gluconolactone-6-phosphate, with NADP⁺ simultaneously accepting hydrogen to form NADPH, thereby maintaining NADPH levels in cells.

Spezifikationen

Contaminants: <0.001% CK, <0.01% GR, HK, 6-PGDH, and PGluM each, <0.002% PGI each, <0.002% PGI

Approximately 350U/mg at +25°C with glucose-6-phosphate as the substrate.

Physikalische Form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Angaben zur Herstellung

Activator: Mg²⁺ (5 to 10 mM)

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash