MABN2470

Anti-Aquaporin-4 Antibody, clone mCCD

clone mCCD, from mouse

• Synonym(e): AQP-4, Mercurial-insensitive water channel, MIWC, WCH4
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: mCCD, monoclonal
• Speziesreaktivität: human, rat, mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotyp: IgG3κ
• NCBI-Hinterlegungsnummer: NP_001641
• UniProt-Hinterlegungsnummer: P55087
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... AQP4(361)

Beschreibung

Allgemeine Beschreibung

Aquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AGP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496).

Spezifität

Clone mCCD specifically detects Aquaporin-4 in human, mouse, and rat brain tissue sections. It targets an epitope with in 71 amino acids from the cytoplasmic domain from the C-terminal region.

Immunogen

Epitope: cytoplasmic domain

His-tagged full length human recombinant Aquaporin-4.

Anwendung

Research Category
Neurowissenschaft

Anti-Aquaporin-4, clone mCCD Antibody, Cat. No. MABN2470, is a highly specific mouse monoclonal antibody that targets Aquaporin-4 and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Western Blotting Analysis: A representative lot detected Aquaporin-4 in Western Blotting applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Aquaporin-4 in Immunoprecipitation applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Immunocytochemistry Analysis: A representative lot detected Aquaporin-4 in Immunocytochemistry applications (Hinson, S.R., et. al. (2017). Proc Natl Acad Sci U S A. 114(21):5491-5496).

Qualität

Evaluated by Immunohistochemistry in rat brain frozen tissue sections.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Aquaporin-4 in rat brain frozen tissue sections.

Zielbeschreibung

34.83 kDa calculated.

Physikalische Form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG3 in PBS without preservatives.

Lagerung und Haltbarkeit

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable