CBA098

InnoCyte Monocyte Cell Migration Assay

• Synonym(e): Monocyte Migration Assay
• UNSPSC-Code: 12352207
• NACRES: NA.32

Eigenschaften

• Verwendung: sufficient for 96 tests
• Qualitätsniveau: 100
• Verpackung: pkg of 1 96-well plate(s)
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze
• Aufnahme: sample type monocyte(s)
• Nachweisverfahren: fluorometric
• Versandbedingung: wet ice

Beschreibung

Allgemeine Beschreibung

The InnoCyte Monoctye Cell Migration Assay is designed to study the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines.

Komponenten

96-Well Monocyte Cell Migration Chamber, Black, Conical 96-Well Plate, D-PBS, Calcein-AM, and a user protocol.

Warnhinweis

Toxicity: Irritant (B)

Prinzip

The Calbiochem InnoCyte 96-Well Monoctye Cell Migration Assay is intended for the study of the effects of various drugs or agents on monocyte motility or for identifying chemoattractant molecules. The assay is not intended for adherent cell lines, such as fibroblasts or solid tumor cell lines. To study these cell types, please use Cat. No. CBA010 or CBA017.

Angaben zur Herstellung

Cell Labeling Mixture: Warm the D-PBS and Calcein-AM Solution to room temperature. Add 35 µl Calcein-AM Solution to 10 ml D-PBS. Mix well. Note: Prepare just before use (see step 7 of the Detailed Protocol below).

Lagerung und Haltbarkeit

Upon arrival store the Calcein-AM at -20°C and the remaining components at 4°C.

Sonstige Hinweise

Cravens, P.D. 2007. Scan. J. Immunol.65, 514.
Charo, I.F., et al. 2006. N. Engl. J. Med.354, 610.
Knitscher, U., et al. 2000. Eur. J. Pharmacol.401, 259.
Turner, L., et al. 1999. Eur. J. Immunol.29, 2288.
Cross, A.K., et al. 1997. Cytokine9, 521.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10-13 - German Storage Class 10 to 13