368067-M

Granzyme B Substrate VIII, Colorimetric

A colorimetric substrate for granzyme B (kcat/Km = 6.6 x 10⁴ M⁻¹s⁻¹).

• Synonym(e): Granzyme B Substrate VIII, Colorimetric, Ac-IEPD- pNA
• Empirische Formel (Hill-System): C₂₈H₃₈N₆O₁₁
• Molekulargewicht: 634.63
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 2826717

Eigenschaften

• Qualitätsniveau: 100
• Assay: ≥95% (HPLC)
• Form: lyophilized solid
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; protect from light
• Farbe: white
• Löslichkeit: DMSO: 5 mg/mL
• Lagertemp.: −20°C
• SMILES String: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)C

Beschreibung

Allgemeine Beschreibung

A colorimetric substrate for granzyme B (k_cat/K_m = 6.6 x 10⁴ M^-1s^-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M^-1cm^-1).

A colorimetric substrate for granzyme B (k_cat/K_m = 66,600 M_-1s_-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M_-1cm_-1).

Biochem./physiol. Wirkung

Primary Target
Colorimetric substrate for granzyme B

kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B

Warnhinweis

Toxicity: Standard Handling (A)

Sequenz

Ac-Ile-Glu-Pro-Asp-pNA

Rekonstituierung

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Sonstige Hinweise

Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable