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Merck

368044

Sigma-Aldrich

Granzyme A, Human, Recombinant, E. coli

Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.

Synonym(e):

GzmA

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About This Item

EC-Nummer:
UNSPSC-Code:
12352202
NACRES:
NA.77

Rekombinant

expressed in E. coli

Qualitätsniveau

Form

liquid

Spezifische Aktivität

≥100 units/μg protein

Hersteller/Markenname

Calbiochem®

Lagerbedingungen

OK to freeze
avoid repeated freeze/thaw cycles

Versandbedingung

wet ice

Lagertemp.

−70°C

Allgemeine Beschreibung

Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
Recombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.

Physikalische Form

In 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.

Sonstige Hinweise

Fan, Z., et al. 2002. Mol. Cell. Biol.22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol.20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem.276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol.244, 68.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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