208706

Ca²⁺/Calmodulin Kinase II, α-Subunit, Rat, Recombinant, Spodoptera frugiperda

Recombinant, rat Ca2+/calmodulin kinase II α-subunit consisting of amino acids 1-325 expressed in S. frugiperda insect cells.

• Synonym(e): CaM Kinase II
• UNSPSC-Code: 12352202

Eigenschaften

• Rekombinant: expressed in insect cells (S. frugiperda)
• Qualitätsniveau: 100
• Assay: ≥90% (SDS-PAGE)
• Form: liquid
• Spezifische Aktivität: ≥250,000 units/mL
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Fremdaktivität: Phosphatases, proteases, none detected
• Versandbedingung: wet ice
• Lagertemp.: −70°C

Beschreibung

Allgemeine Beschreibung

Recombinant, rat Ca²⁺/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. CaM kinase II is a serine/threonine kinase that phosphorylates a wide variety of intracellular proteins. Reportedly involved in the modulation of neurite growth by cAMP. Lower levels of CaM kinase II a-subunit predispose neurons to ischemic damage.

Recombinant, rat Ca²⁺/calmodulin kinase II α-subunit consisting of amino acids 1-325 (corresponding to monomeric α-subunit) expressed in S. frugiperda insect cells. Reportedly involved in the modulation of neurite outgrowth by cAMP. Lower levels of CaM kinase II α-subunit predispose neurons to ischemic damage.

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to Autocamtide-2 (Cat. No. 189475), KKALRRQETVDAL, per min at 30°C, pH 7.5.

Physikalische Form

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 0.1 mM EDTA, 50% glycerol, 0.02% Tween^® 20 Detergent, pH 7.5.

Rekonstituierung

Following initial thaw, aliquot and freeze (-70°C).

Sonstige Hinweise

Tashima, K., et al. 1996. J. Neurochem. 66, 57.
Waxham, M.N., et al. 1996. J. Cereb. Flow Metab. 16, 1.
Hanson, P.I., and Schulman, H. 1992. Annu. Rev. Biochem. 61, 559.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1