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Merck

05-389-AF488

Sigma-Aldrich

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor 488 Conjugate

clone 23A8, from mouse, ALEXA FLUOR 488

Synonym(e):

Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1, Rac1

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Biologische Quelle

mouse

Qualitätsniveau

Konjugat

ALEXA FLUOR 488

Antikörperform

purified antibody

Antikörper-Produkttyp

primary antibodies

Klon

23A8, monoclonal

Speziesreaktivität

rat, mouse, human

Methode(n)

immunocytochemistry: suitable

Isotyp

IgG2b

NCBI-Hinterlegungsnummer

UniProt-Hinterlegungsnummer

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

human ... RAC1(5879)

Allgemeine Beschreibung

Ras-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions, including cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family consists of 22 members, which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamily (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.

Spezifität

Recognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.

Immunogen

Recombinant protein containing the full length human Rac.

Anwendung

Research Category
Zelluläre Signaltransduktion
Research Sub Category
Entwicklungsabhängige Signalübertragung
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Rac1 in NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
This Anti-Rac1 Antibody, clone 23A8, Alexa Fluor 488 Conjugate is validated for use in Immunocytochemistry for the detection of Rac1.

Qualität

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in A431 cells.

Zielbeschreibung

~21 kDa observed

Physikalische Form

Protein G Purified
Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Rechtliche Hinweise

ALEXA FLUOR is a trademark of Life Technologies

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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