Bovine Serum Albumin (BSA) Blocking Buffer Recipe

Bovine serum albumin (BSA) blocking buffer is ideal for saturating excess protein-binding sites on membranes and microplates for Western blotting and ELISA applications, respectively. Typically, 1-3% BSA is sufficient for most applications. To make 100 mL of a 1% BSA blocking buffer, dissolve 1 g of BSA in 100 mL of TBST.

The BSA blocking buffer recipe calculator enables the accurate preparation of BSA blocking solution whether you are making enough for a single experiment or for the entire lab. Input your desired concentration and volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.

BSA Blocking Buffer Recipe Calculator

OTHER RECIPE CALCULATORS FOR WESTERN BLOTTING BUFFERS & SOLUTIONS

Stock Solutions

• 4X SDS Sample Loading Buffer
• 10X Phosphate-Buffered Saline (PBS)
• 10X Tris-Buffered Saline (TBS) Stock Solution
• Stock Components for Western Blot Stripping Solutions

Wash & Blocking Buffers

• 1X Tris-Buffered Saline (TBS) Working Solution
• 1X Tris-Buffered Saline, 0.1% Tween® 20 Detergent (TBST)
• 1X PBS (Phosphate-Buffered Saline)
• 1X Phosphate-Buffered Saline, 0.1% Tween® 20 Detergent (PBST)
• Non-Fat Dry Milk (NFDM) Blocking Buffer

Stripping Solutions

• Detergent-based Western Blot Stripping Buffer
• Acidic Western Blot Stripping Solution
• Stock Components for Western Blot Stripping Solutions