PECAS9
PURedit® Cas9 Protein
from Streptococcus pyogenes, recombinant, expressed in E. coli, 3X NLS
Synonym(s):
Cas9, SpCas9, SpyCas9
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.51
Recommended Products
recombinant
expressed in E. coli
Quality Level
assay
≥95% (SDS-PAGE)
form
lyophilized powder
packaging
pkg of 1 kit (4 components)
shipped in
wet ice
storage temp.
−20°C
General description
Recombinant PURedit Cas9 protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs, Streptococcus pyogenes PURedit Cas9 protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
PURedit translational-grade CRISPR reagents are engineered to meet superior manufacturing standards that enable consistent, high-quality performance every time.
Application
Functional Genomics; Target Validation; Genome Editing
Features and Benefits
- High Quality for preclinical and early clinical research
- Highest performance efficiency of any Cas9 variant
- Ready to inject/transfect
Packaging
pkg of 50 μg (≥ 300 pmol)
pkg of 250 μg (≥ 1500 pmol)
pkg of 250 μg (≥ 1500 pmol)
Components
Each kit consists of:
- one vial of PURedit Cas9 Recombinant Protein
- one vial of PURedit Transfection Buffer
- one vial of Reconstitution Buffer
- one vial of Dilution Buffer
Principle
CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.
Reconstitution
Lyophilized PURedit Cas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube.
Other Notes
Use our CRISPR Selection Tool to order gRNA
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRproteins
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
PURedit is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
Guaranteed PURedit™ CRISPR synthetic gRNAs and Cas9 protein offer industry-leading on-site cutting and specificity
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