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Key Documents

MBD0038

Sigma-Aldrich

Fungal DNA standard from Aspergillus fumigatus

Suitable for PCR, sequencing and NGS

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.24

Quality Level

200

form

liquid

concentration

10 ng/μL

technique(s)

FISH: suitable

shipped in

ambient

storage temp.

−20°C

General description

Standardization of sample analysis is essential in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing, and bioinformatics analyses.1 The human microbiome includes not only bacteria, but also viruses and fungi. While the “bacteriome” field has been researched extensively in the past years, fungi, comprising the “mycobiome”, remain relatively neglected. Fungi and yeasts have been shown to be involved in illness conditions such as cancer and gastrointestinal disease. The human gut is populated by three fungal phyla, Ascomycota, Basidiomycota, and Zygomycota.

The fungal microbial genomic DNA standard from Aspergillus fumigatus can serve as standard for benchmarking the performance along the workflow of microbiome research and metagenomic analyses, as well as a tool to increase reproducibility and allow comparison of results obtained by different labs.

Aspergillus fumigatus is an ubiquitous saprotroph fungus belonging to Ascomycota. Aspergillus fumigatus spores can induce allergic airways response after antibiotic treatment2. With increases in the number of immunosuppressed patients, there has been a dramatic increase in severe and usually fatal invasive aspergillosis, caused by the conidia of Aspergillus species. Aspergillosis is now the most common mold infection worldwide.3

The genomic DNA is provided at ≥10 ng/μL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles with this product.


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable as a standard for PCR, Sanger and next generation sequencing (NGS).

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, Sanger sequencing and 18S NGS
  • Improves bioinformatics analyses
  • Increases reproducibility and repeatability
  • Identity and purity of the genomic Aspergillus fumigatus DNA has been confirmed by 18S NGS and gel electrophoresis

Physical form

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

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Articles

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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