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L1882

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

Synonym(s):

WGA, Wheat germ agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

conjugate

agarose conjugate

Quality Level

200

form

saline suspension

extent of labeling

5-10 mg per mL

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

capacity

≥0.1 mg/mL binding capacity (ovomucoid)

storage temp.

2-8°C

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General description

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Application

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)2 or N-acetylneuraminic acid.

Biochem/physiol Actions

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Physical form

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Preparation Note

Conjugates are prepared from affinity purified lectin.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

ppe

Eyeshields, Gloves

Certificates of Analysis (COA)

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K Paz et al.
The Journal of biological chemistry, 271(12), 6998-7003 (1996-03-22)
A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM)
Binding sugars: from natural lectins to synthetic receptors and engineered neolectins
Arnaud J, et al.
Chemical Society Reviews, 42(11), 4798-4813 (2013)

Articles

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Performing a Purification of Glycoproteins and Polysaccharides with Agarose Wheat Germ Lectin

This page shows how to purify glycoproteins and polysaccharides by affinity chromatography using Agarose Wheat Germ Lectin.

Converting From Linear Flow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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