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Key Documents

P4244

Sigma-Aldrich

Phosphate buffered saline

liquid, pH 7.2 (25 °C), autoclaved

Synonym(s):

PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate buffered saline, Autoclaved, pH 7.2 (25 °C)

Quality Level

200

description

Sodium chloride, 150 mM, and sodium phosphate, 150 mM

form

liquid

pH

7.2 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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General description

Phosphate buffered saline (PBS) is an isotonic standard buffer solution. PBS imitates the pH, ion concentrations, and osmolarity of the human body. It serves as an ideal buffer for maintaining the osmolarity and physiological pH for biomedical dilutions in cell culture and protein chemistry. PBS is used for standard enzymatic tests with active metal-organic framework (MOF) biocomposites. PBS is considered non-toxic to cells.

Application

Phosphate buffered saline has been used:
  • for washing bacterial cells after harvesting by centrifugation
  • for washing the peritoneal cavity for isolation of peritoneal macrophages
  • as a control and to dilute phytohemagglutinin (PHA) to prepare subcutaneous injections and study the immune response in birds.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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