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G001593

HPLC Analysis of Peptides on Discovery® BIO Wide Pore C18 (Gradient #2)

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Discovery BIO Wide Pore C18, 15 cm × 4.6 mm I.D., 5 μm particles (568222-U)
mobile phase: [A] (80:20), 0.1%TFA in water:0.1% TFA in acetonitrile; [B] (66:34), 0.1% TFA in water:0.1% TFA in acetonitrile
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 5 μL Sigma Peptide Mix P2693 (~25 μg ea. Peptide) (~25 μg ea. peptide)
sample: Sigma Peptide Mix (Cat. No. P2693)

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Bombesin
    Luteinizing hormone releasing hormone
    Oxytocin

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