H9037

Anti-HA−TRITC antibody, Mouse monoclonal

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
• MDL number: MFCD00803873
• UNSPSC Code: 12352203
• NACRES: NA.56

Properties

• biological source: mouse
• conjugate: TRITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• concentration: ~1 mg/mL
• technique(s): direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Specificity

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable