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CRISPR13H

Sigma-Aldrich

CRISPR-Lenti Human HPRT1 Positive Control High Titer Transduction Particles

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

form

liquid

Quality Level

200

packaging

vial of 200 μL

concentration

5x108  VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

Application

Functional Genomics/Screening/Target Validation
  • Positive control, HPRT1, CRISPR high titer lentivirus format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crispr.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.

Other Notes

This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL−2) organisms in laboratory handling. Follow all published RGL−2 guidelines for laboratory handling and waste decontamination.

Physical form

200 μl virus, minimum p24 titer > 5 x 108 TU/mL

Legal Information

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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