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T1283

Sigma-Aldrich

holo-Transferrin bovine

Iron-saturated, BioReagent, suitable for cell culture

Sinónimos:

Siderophilin, Siderophilin, iron-saturated

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About This Item

Número de CAS:
11096-37-0
EC Number:
234-318-8
MDL number:
MFCD00131337
UNSPSC Code:
12352202
NACRES:
NA.75

product line

BioReagent

assay

97-100% (agarose gel electrophoresis)

form

powder

concentration

~25 mM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

Q29443

shipped in

ambient

storage temp.

2-8°C

Gene Information

bovine ... TF(280705)

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Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Bovine holo-transferrin can be used with a wide range of cells types. Bovine holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Analysis Note

Purity by agarose gel electrophoresis.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Han Hu et al.
Animal science journal = Nihon chikusan Gakkaiho, 87(1), 84-91 (2015-10-16)
In this study, bovine mammary epithelial cells were used to study stress responses after cells were exposed to 42°C for 0.5, 1, 3, 5, 8 or 12 h, and 38°C as control. The transcription of the genes (HSP27, HSP70 and
William Schachterle et al.
Nature communications, 8, 13963-13963 (2017-01-17)
Transplanting vascular endothelial cells (ECs) to support metabolism and express regenerative paracrine factors is a strategy to treat vasculopathies and to promote tissue regeneration. However, transplantation strategies have been challenging to develop, because ECs are difficult to culture and little
Wenting Dai et al.
The British journal of nutrition, 123(5), 489-498 (2019-11-13)
Despite the well-characterised mechanisms of amino acids (AA) regulation of milk protein synthesis in mammary glands (MG), the underlying specific AA regulatory machinery in bovine MG remains further elucidated. As methionine (Met) is one of the most important essential and
Guihua Du et al.
Development (Cambridge, England), 147(8) (2020-03-20)
Continual spermatogenesis relies on the actions of an undifferentiated spermatogonial population that is composed of stem cells and progenitors. Here, using mouse models, we explored the role of RNA-binding proteins (RBPs) in regulation of the biological activities of this population.
Francis Biville et al.
PloS one, 9(10), e107612-e107612 (2014-10-08)
The ability to acquire iron from various sources has been demonstrated to be a major determinant in the pathogenesis of Neisseria meningitidis. Outside the cells, iron is bound to transferrin in serum, or to lactoferrin in mucosal secretions. Meningococci can
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Artículos

Transferrin in Cell Culture

How transferrin and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of transferrin.

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