SAE0152
Lisozima from chicken egg white
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, protein ≥90%, ≥40,000 units/mg protein
Sinónimos:
Mucopéptido N-acetilmuramoilhidrolasa, Muramidasa
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About This Item
Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.77
Productos recomendados
biological source
chicken egg white
Quality Level
form
powder
specific activity
≥40000 units/mg protein
mol wt
single-chain 14.3 kDa
feature
DNA free
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
cell analysis
shipped in
wet ice
storage temp.
−20°C
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General description
Purified Lysozyme DNA free SAE0152 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges.1 It hydrolyzes b(1à 4) linkages between N-acetylmuraminic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin.2,3,4 The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan.
The enzyme is active over a broad pH range (6.0-9.0).
Lysozyme activity: ≥40,000 units/mg protein.
For isolation of nucleic acids, lysozyme has been used in the lysis peptidoglycan layer of bacterial cell walls.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential. This purified lysozyme preparation (SAE0152) is purified from chicken egg white, crystallized three times, dialyzed, and supplied as a lyophilized powder. Protein content by UV absorbance is ≥ 90% with the remainder (∼10%) being buffer salts such as sodium acetate and sodium chloride, and undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Application
La enzima rompe las paredes celulares de las bacterias; se utiliza para preparar esferoplastos.
- Lysozyme is used for the extraction of genomic DNA from bacterial cells.
- Lysozyme is used as an external standard for MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass analysis.
- Lysonzyme is used to prepare spheroplasts.
Biochem/physiol Actions
La lisozima hidroliza los enlaces β(1→4) entre los restos del ácido N-acetilmurámico y los restos de N-acetil-D-glucosamina en el peptidoglucano y entre los residuos de N-acetil-D-glucosamina en la quitodextrina. Las células grampositivas son bastante sensibles a esta hidrólisis, ya que sus paredes celulares tienen una elevada proporción de peptidoglucanos. Las bacterias gramnegativas son menos sensibles debido a la presencia de una membrana externa y a una menor proporción de peptidoglucanos. Sin embargo, estas células pueden ser hidrolizadas en presencia de EDTA que forma complejos con los iones metálicos en la membrana bacteriana externa.
La enzima es activa en un amplio intervalo de pH (de 6,0 a 9,0). A pH 6,2, se observa actividad máxima en un intervalo de fuerzas iónicas más amplio (de 0,02 a 0,100 M) que a pH 9,2 (de 0,01 a 0,06 M).
La enzima es activa en un amplio intervalo de pH (de 6,0 a 9,0). A pH 6,2, se observa actividad máxima en un intervalo de fuerzas iónicas más amplio (de 0,02 a 0,100 M) que a pH 9,2 (de 0,01 a 0,06 M).
La lisozima hidroliza los enlaces β(1→4) entre los restos del ácido N-acetilmurámico y los restos de N-acetil-D-glucosamina en el peptidoglucano y entre los residuos de N-acetil-D-glucosamina en la quitodextrina. Las células grampositivas son bastante sensibles a esta hidrólisis, ya que sus paredes celulares tienen una elevada proporción de peptidoglucanos.
Features and Benefits
Purified Lysozyme free SAE0152 undergoes strict quality control testing to ensure it will be Free of DNA contaminants, suitable for Microbiome research.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
The study of microbial communities has been revolutionized in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. Since DNA contamination during sample preparation is a major problem of these sequence-based approaches, DNA extraction reagents free of DNA contaminates are essential.
Unit Definition
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Enzymes provide a non-mechanical method for cell lysis and protoplast preparation. It may seem like a simple process to throw in your enzyme, stick your tube in the water-bath and walk away, but what is actually going on in that process?
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