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Key Documents

S2263

Sigma-Aldrich

Sheep Serum

USA origin, sterile-filtered, suitable for cell culture

Sinónimos:

ovine sera, ovine serum, sheep sera

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

biological source

sheep

Quality Level

500

sterility

sterile-filtered

composition

hemoglobin, ≤50 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤100 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Application

Sheep serum is useful for the propagation of long-term cultures of erythrocytic stages of the Theileria uilenbergi intracellular protozoan parasite.

Preparation Note

Collected from adult animals.

Analysis Note

Endotoxin tested

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

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Daigo Inoue et al.
PloS one, 6(5), e19713-e19713 (2011-05-24)
For the detection and sub-cellular (co)-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval
Rohola Hosseini et al.
Autophagy, 10(10), 1844-1857 (2014-08-16)
High-resolution imaging of autophagy has been used intensively in cell culture studies, but so far it has been difficult to visualize this process in detail in whole animal models. In this study we present a versatile method for high-resolution imaging
Cassandra D Kinch et al.
Aquatic toxicology (Amsterdam, Netherlands), 175, 286-298 (2016-04-24)
Exposure to environmental contaminants has been linked to developmental and reproductive abnormalities leading to infertility, spontaneous abortion, reduced number of offspring, and metabolic disorders. In addition, there is evidence linking environmental contaminants and endocrine disruption to abnormal developmental rate, defects
Mardi S Byerly et al.
PloS one, 8(11), e80781-e80781 (2013-12-07)
During development, prenatal and postnatal factors program homeostatic set points to regulate food intake and body weight in the adult. Combinations of genetic and environmental factors contribute to the development of neural circuitry that regulates whole-body energy homeostasis. Brain-derived neurotrophic
Joana P G Miranda et al.
American journal of veterinary research, 67(11), 1908-1913 (2006-11-03)
To establish optimal conditions for long-term culture of the erythrocytic stage of Theileria uilenbergi. Red blood cells from 3 splenectomized sheep experimentally infected with a blood stabilate of T uilenbergi. Cultures of T uilenbergi were initiated by use of blood
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Protocolos

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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