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MilliporeSigma

P6282

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinónimos:

PDL HBr

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75

product name

Poli-L-lisina hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

solubility

H2O: soluble 50 mL/vial, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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General description

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Application

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.

Biochem/physiol Actions

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Components

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Caution

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Related product

Referencia del producto
Descripción
Precios

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Kasemeier KJC, et al.
The Journal of Neuroscience, 30(39), 13078-13088 (2010)
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Artículos

Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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