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P5899

Sigma-Aldrich

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinónimos:

PDL HBr

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About This Item

Número de CAS:
25988-63-0
MDL number:
MFCD00237230
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
24898718
NACRES:
NA.75

product name

Poli-L-lisina hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

biological source

synthetic (organic)

Quality Level

200

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Application

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation
  • T75 flasks for primary astrocytic cultures

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Components

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.

Caution

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Malgorzata J Podolska et al.
Journal of leukocyte biology, 106(6), 1359-1366 (2019-09-04)
The release of neutrophil extracellular traps (NETs) is one of the weapons neutrophils have in their armory. NETs consist of extracellular chromatin fibers decorated with a plethora of cytoplasmic and granular proteins, such as the antimicrobial serine protease neutrophil elastase
Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Skye E Smith et al.
eNeuro, 7(4) (2020-08-02)
Aberrant migration of inhibitory interneurons can alter the formation of cortical circuitry and lead to severe neurologic disorders including epilepsy, autism, and schizophrenia. However, mechanisms involved in directing the migration of interneurons remain incompletely understood. Using a mouse model, we
Stuart B Mazzone et al.
Molecular neurobiology, 57(2), 949-963 (2019-10-21)
Bronchopulmonary sensory neurons are derived from the vagal sensory ganglia and are essential for monitoring the physical and chemical environment of the airways and lungs. Subtypes are heterogenous in their responsiveness to stimuli, phenotype, and developmental origin, but they collectively
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Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolos

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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