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P4300

Sigma-Aldrich

Pectinase from Rhizopus sp.

suitable for plant cell culture, BioReagent, crude powder

Sinónimos:

Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase

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About This Item

Número de CAS:
9032-75-1
Comisión internacional de enzimas:
3.2.1.15 (BRENDA, IUBMB)
EC Number:
232-885-6
MDL number:
MFCD00131809
UNSPSC Code:
12352200

product line

BioReagent

form

crude powder

specific activity

400-800 units/g solid

technique(s)

cell culture | plant: suitable

storage temp.

−20°C

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General description

Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Application

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Katsuichi Saito et al.
Microbiological research, 159(1), 83-86 (2004-05-27)
The pectinolytic enzyme from the solid-state culture of Rhizopus oryzae NBRC 4707 was purified to homogeneity by column chromatography on CM-Toyopearl 650 M and hydroxylapatite. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 31,000
Shigenobu Yoshida et al.
Mycological research, 108(Pt 12), 1407-1414 (2005-03-11)
The polygalacturonase (PG)-encoding gene (rpg1) of Rhizopus oryzae, the causal pathogen of rhizopus rot of mulberry, was cloned and sequenced. PGs were partially purified from incubation mixture of 2% pectin medium and their N-terminal amino acid sequences were determined by
K Saito et al.
Journal of industrial microbiology & biotechnology, 30(7), 440-444 (2003-07-05)
Rhizopus oryzae strain NBRC 4707 produced lactic acid and ethanol more efficiently than strain NRRL 395 in potato pulp, an agricultural by-product of the starch industry. The two strains developed comparable activities of xylanase, cellulase, alpha-amylase, and glucoamylase, while the
Jeffrey A Mertens et al.
Fungal genetics and biology : FG & B, 45(12), 1616-1624 (2008-10-22)
A search of the recently sequenced Rhizopus oryzae strain 99-880 genome database uncovered 18 putative polygalacturonase genes with two genes being identical and only one with similarity to a previously reported R. oryzae polygalacturonase gene. The 17 different genes share
Claudia Schinke et al.
International microbiology : the official journal of the Spanish Society for Microbiology, 15(1), 1-7 (2012-07-28)
Macrophomina phaseolina, phylum Ascomycota, is a phytopathogenic fungus distributed worldwide in hot dry areas. There are few studies on its secreted lipases and none on its colony radial growth rate, an indicator of fungal ability to use nutrients for growth
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