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MilliporeSigma

N2133

Sigma-Aldrich

Neuraminidase from Clostridium perfringens (C. welchii)

Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)

Sinónimos:

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type X

Quality Level

form

lyophilized powder

specific activity

≥50 units/mg protein (using 4MU-NANA)

storage temp.

−20°C

Gene Information

Clostridium perfringens str. 13 ... nanI(988807)

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General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Clostridium perfringens has been used in a study to assess purification via affinity chromatography. It has also been used in a study to investigate site-directed mutations of amino acids of the neuraminidase gene, nanH.

Biochem/physiol Actions

Neuraminidase can block attachment of type 3 reovirus to cell membranes. This effect is related to the ability of neuraminidase to hydrolysis sialic acid residues within cell surface receptors.
Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.
Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.
The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Unit Definition

One unit will hydrolyze 1.0 micromole of 2′-(4-Methylumbelliferyl)-alpha-D-N-actetylneuraminic acid per min at pH 5.0 at 37 °C.

Preparation Note

Purified by affinity chromatography from Type VIII (N 5631)

Analysis Note

Package sizes based on 4MU-NANA units
Package sizes based on the 4MU-NANA units

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Purification of neuraminidases from Vibrio Cholerae, Clostridium Perfringens and influenza virus by affinity chromatography.
P Cuatrecasas et al.
Biochemical and biophysical research communications, 44(1), 178-184 (1971-07-02)
J Shemer et al.
Diabetologia, 29(5), 321-329 (1986-05-01)
Specific insulin receptors are present in the liver and brain of the lizard Anolis carolinesis. In this study, the specific binding of 125I-insulin to the receptors showed time, temperature and pH dependency. Specific binding to crude membranes prepared from brain
L V Gubareva et al.
Lancet (London, England), 355(9206), 827-835 (2000-03-11)
Neuraminidase promotes influenza virus release from infected cells and facilitates virus spread within the respiratory tract. Several potent and specific inhibitors of this enzyme have been developed, and two (zanamivir and oseltamivir) have been approved for human use. Unlike amantadine
C H Chien et al.
Enzyme and microbial technology, 19(4), 267-276 (1996-09-01)
The small nanH gene encoding the neuraminidase from Clostridium perfringens ATCC 10543 was cloned in JM109 using pUC19 as a vector. Sequence analysis revealed an ORF encoding 382 amino acids without a signal peptide sequence. Four regions of amino-acid sequence
Marjorie E Kanof
Current protocols in immunology, Chapter 7, 7-7 (2008-04-25)
This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the

Protocolos

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

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