N3786
α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens
Proteomics Grade, suitable for MALDI-TOF MS
Sinónimos:
Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
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About This Item
Productos recomendados
form
lyophilized powder
Quality Level
quality
Proteomics Grade
specific activity
≥25 U/vial
suitability
suitable for MALDI-TOF MS
storage temp.
2-8°C
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Application
Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.
Packaging
Provided with 5× concentrate reaction buffer.
Quality
The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.
Unit Definition
One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.
Physical form
Lyophilized powder
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- N3536Neuraminidase 5X Reaction Buffer 1.5 mLSDS
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Referencia del producto
Descripción
Precios
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Glycan Sequencing Using Exoglycosidases
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