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Key Documents

GE28-4017-47

GSTrap 4B

Cytiva 28-4017-47, pack of 1 × 5 mL

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About This Item

UNSPSC Code:
23151816
NACRES:
NA.56

ligand

glutathione

packaging

pack of 1 × 5 mL

manufacturer/tradename

Cytiva 28-4017-47

parameter

42 psi (H2O at 25°C)

bed size

16 mm × 25 mm

bed volume

5 mL

column I.D.

16 mm

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

4-13

working range

4-13

capacity

>25 mg binding capacity (horse liver GST/ml medium)

General description

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Application

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Features and Benefits

  • High binding capacity.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

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