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Nucleasas Benzonase®

≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

Sinónimos:

Endonucleasas from Serratia marcescens

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About This Item

Número de CAS:
9025-65-4
Comisión internacional de enzimas:
3.1.30.2 (BRENDA, IUBMB)
MDL number:
MFCD00131010
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Serratia marcescens

Quality Level

200

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

30 kDa

concentration

≥250 units/μL

application(s)

research use

foreign activity

protease, essentially free

shipped in

wet ice

storage temp.

−20°C

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General description

La nucleasa Benzonase® es una endonucleasa genotecnológica procedente de Serratia marcescens. Este dímero proteico con dos enlaces disulfuro esenciales es capaz de atacar y degradar todas las formas de ADN y ARN (monocatenarias, bicatenarias, lineales y circulares) en una amplia variedad de condiciones de funcionamiento. Puede digerir por completo los ácidos nucleicos a oligonucleótidos de 3 a 5 bases de longitud terminados en 5′-monofosfato, lo que la convierte en la herramienta ideal para separar los ácidos nucleicos de las proteínas recombinantes y para aplicaciones que requieran una digestión completa de los ácidos nucleicos. Además de reducir la viscosidad en los extractos de proteínas y evitar la aglutinación celular, el pretratamiento de muestras proteicas con las nucleasas Benzonase® puede mejorar significativamente su resolución en la electroforesis bidimensional en gel al eliminar cualquier ácido nucleico unido. Esta enzima versátil puede digerir el ADN y el ARN nativos o desnaturalizados por calor con su pH de actividad enzimática óptimo que oscila entre 8,0 y 9,2. Elija las nucleasas Benzonase® por su capacidad de eliminación de los ácidos nucleicos y de aumento de la pureza y la calidad de las muestras proteicas.

Application

Benzonase® Nuclease has been used: as a component in ice-cold lysis buffer C to digest DNA and  RNA to facilitate the complete release of all nuclear proteins in the immunoprecipitation step to release protein complexes from the nucleoplasm and chromatinas a supplement in RIPA to fractionate SHSY5Y cells for immunoprecipitation to remove residual nucleic acids from the aortic roots in decellularization method
Utilizado para eliminación de los ácidos nucleicos de las muestras proteicas.

Biochem/physiol Actions

Benzonase® Nuclease can completely digest nucleic acids into 5′-monophosphate terminated oligonucleotides of 3 to 5 bases in length, making it the ideal tool for removing nucleic acids from recombinant proteins and for applications that require complete digestion of nucleic acids. In addition to reducing viscosity in protein extracts and preventing cell clumping, pretreatment of protein samples with Benzonase® nuclease can significantly improve their resolution on 2D gel electrophoresis by eliminating any bound nucleic acids. This versatile enzyme can digest both native or heat-denatured DNA and RNA, with its optimum pH for enzyme activity found to be 8.0-9.2. Benzonase® nuclease is effective at removing host DNA from microbiome samples. In many cases, microbiome samples (such as saliva or skin) will have a high percentage of host DNA that interferes with downstream results. Our experts show that the reduction of host DNA lowers the cost of sequencing while increasing and improving the data. Experimental data is shown in the technical article - Benzonase® Nuclease for Microbiome Workflows
Digiere el ADN y el ARN nativos o desnaturalizados por calor.

Features and Benefits

Eliminación del ADN del huésped en muestras de microbioma

Digestión eficaz del ácido nucleico en una variedad de procedimientos de trabajo

Reducción de la viscosidad durante la extracción de proteínas

Unit Definition

Una unidad digerirá el ADN de esperma de salmón tratado con ultrasonidos a oligonucleótidos solubles en ácido equivalentes a ΔA260 de 1,0 en 30 minutos a pH 8,0 a 37 °C (volumen de reacción 2,625 ml).

Physical form

Disolución en glicerol al 50% que contenga Tris HCl 20 mM, pH 8,0, MgCl2 2 mM y NaCl 20 mM.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

La nucleasa Benzonase® es suministrada por Merck KGaA, Darmstadt, Alemania y sus filiales.
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Jos J M Drabbels et al.
Blood, 118(19), e149-e155 (2011-09-21)
Microchimerism is defined by the presence of low levels of nonhost cells in a person. We developed a reliable method for separating viable microchimeric cells from the host environment. For flow cytometric cell sorting, HLA antigens were targeted with human
Janus S Jakobsen et al.
Science advances, 5(7), eaaw4304-eaaw4304 (2019-07-17)
The key myeloid transcription factor (TF), CEBPA, is frequently mutated in acute myeloid leukemia (AML), but the direct molecular effects of this leukemic driver mutation remain elusive. To investigate CEBPA mutant AML, we performed microscale, in vivo chromatin immunoprecipitation sequencing
T K Ball et al.
Gene, 57(2-3), 183-192 (1987-01-01)
We are studying exoproteins of the enteric bacterium Serratia marcescens as a model system for the release of extracellular proteins from the cell. In this work we report the cloning of the gene for a secreted nuclease from S. marcescens
T K Ball et al.
Nucleic acids research, 20(19), 4971-4974 (1992-10-11)
The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a
An extracellular nuclease from Serratia marcescens. II. Specificity of the enzyme.
M Nestle et al.
The Journal of biological chemistry, 244(19), 5219-5225 (1969-10-10)
Ver todos los artículos relacionados

Artículos

Benzonase® Nuclease for Microbiome Workflows

Benzonase® Nuclease for reducing host DNA in microbiome workflows and enhancing taxa identification.

Benzonase® Nuclease Q&A

This page lists nine frequently asked questions and answers about Benzonase® Nuclease.

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

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