GE17-5079-02

HiTrap^® rProtein A Fast Flow

Cytiva 17-5079-02, pack of 2 × 1 mL

• UNSPSC Code: 23151816
• NACRES: NA.56

Propiedades

• ligand: recombinant protein A (E. coli)
• shelf life: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• packaging: pack of 2 × 1 mL
• manufacturer/tradename: Cytiva 17-5079-02
• storage condition: (20% Ehtanol); (20% Ethanol)
• parameter: <4 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.); 42 psi
• bed size: 7 mm × 25 mm
• bed volume: 1 mL
• column I.D.: 7 mm
• matrix: 4% cross-linked agarose
• particle size: 60-165 μm
• average diameter: 90 μm (d50v)
• cleaning: 2-11(pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.)
• working range: 3-10
• capacity: ~50 mg binding capacity (human lgG/ml medium)(Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.)
• suitability: suitable for bioprocess medium
• storage temp.: 2-8°C

Descripción

General description

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast FLow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

Application

The recombinant Protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast FLow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

Features and Benefits

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established Protein A-based medium used in many approved MAb processes.
• Recombinant Protein A exhibits similar Fc region specificity to that of native Protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA^® design.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Información de seguridad

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids