GE17-0404-03
HiTrap® Protein G High Performance
Cytiva 17-0404-03, pack of 2 × 1 mL
Sinónimos:
HiTrap Protein G, Protein G affinity column
About This Item
Productos recomendados
ligand
recombinant protein G lacking albumin-binding region
form
prepacked column
crosslinking
cross-linked
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 17-0404-03
storage condition
(20% Ehtanol)
(20% Ethanol)
parameter
42 psi
technique(s)
liquid chromatography (LC): suitable
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
size
matrix
(cross-linked agarose, spherical)
matrix active group
Protein G
avg. part. size
34 μm
cleaning
2-9
working range
3-9
capacity
>1 mg binding capacity (lgG/column)
25 mg binding capacity(human IgG/column)
separation technique
affinity
storage temp.
2-8°C
Categorías relacionadas
General description
Features and Benefits
- High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
- 34 μm bead size for high performance, high-resolution purifications.
- Binds broad range of different species and subclasses of IgG.
- Simple and proven method giving reproducible results.
- Designed for use with syringe, peristalic pump, or chromatography system.
Storage and Stability
Analysis Note
Legal Information
related product
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
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Artículos
Antibodies are needed for research and industrial purposes in different quantities, from microgram to kilogram scale. It is important to design and use a purification method that will yield protein of a quality and quantity that is adequate for the particular application.
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Protocolos
General instructions for affinity purification using HiTrap columns from Cytiva.
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