BHK 21 CL13(IZS)

93120815, hamster kidney, Fibroblast

• UNSPSC Code: 41106514

Propiedades

• product name: BHK 21 CL13(IZS), 93120815
• biological source: hamster kidney
• growth mode: Adherent
• karyotype: 2n = 44
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Susceptible to a range of viruses including adenovirus 25, vaccinia, herpes simplex, reovirus 3, vesicular stomatitis, rubella, pseudorabies.

Application

Virus studies

Culture Medium

GMEM + 2mM Glutamine + 10% Tryptose Phosphate Broth + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.