BHK 21 (Clone 13) from hamster

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85011433, hamster kidney, Fibroblast

• Sinónimos: BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
• UNSPSC Code: 41106514

Propiedades

• biological source: hamster kidney
• packaging: tube of 5 μg 85011433-DNA-5UG; pkg of vial of cells 85011433-1VL
• growth mode: Adherent
• karyotype: 2n = 44, pseudodiploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Hamster Syrian kidney

Cell Line Description

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

Application

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.

Virus replication studies

Culture Medium

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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