NG108-15

88112302

• UNSPSC Code: 41106514

Propiedades

• biological source: Mouse x Rat Hybridoma nerve
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse neuroblastoma x Rat glioma hybrid

Cell Line Description

Neuroblastoma x glioma hybrid was formed by Sendai virus-induced fusion of the mouse neuroblastoma clone N18TG-2 and the rat glioma clone C6 BV-1.

Application

Hybridisation, fusion partner and virus studies: Sendai virus

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 seeding at 2x100,000 cells/cm² . Cells adhere lightly, detach by gently knocking the flask do not use trypsin. 5% CO₂; 37°C. The cells do produce large amounts of CO₂, media changes may be necessary.

Other Notes

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