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A1595

Sigma-Aldrich

Seroalbúmina bovina solution

10% in DPBS, low endotoxin, fatty acid free, suitable for cell culture, sterile-filtered

Sinónimos:

Albúmina solution from bovine serum, Disolución BSA

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About This Item

Número de CAS:
9048-46-8
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.27

biological source

bovine

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

assay

90-120 mg/mL protein basis (biuret)

form

solution

purified by

heat shock fractionation

contains

does not contain stabilizer or preservative

origin

USA origin

concentration

10% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤0.05% fatty acid
≤200 EU/mL endotoxin

pH

7.1-7.7

UniProt accession no.

P02769

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Recommended for use as a base for the preparation of defined, fatty acid supplements. Can be loaded with up to 4 moles of fatty acids per mole of albumin.

BSA is a known fatty acid (FA) carrier. Thus in FA-related applications like cell culture, the specific BSA can be important, as related to control over specific fatty acids used to culture cell lines, because different cell lines can be variably sensitive to particular FA;s. Fatty acid-free BSA therefore is useful in cell culture work where the specific fatty acid content must be carefully controlled, so that researchers can control the specific FA′s needed for their particular cell lines. Fatty acid-free BSA also allows for optimal and maximum binding sites for the specific fatty acids that researchers wish to use in their particular cell culture experiments. Use of fatty-acid-free BSA also addresses concerns about endogenous FA′s potentially in non-fatty acid-free BSA.

Features and Benefits

  • Fatty acid free / tested to be low in fatty acid content
  • Low endotoxin
  • Sterile-filtered

Preparation Note

Prepared with Heat Shock Fractionated fatty acid-free albumin in DPBS D5652.

Related product

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Albumin in Cell Culture

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

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