30915

Denhardt′s solution

BioReagent, for molecular biology, lyophilized powder

• MDL number: MFCD00717549
• UNSPSC Code: 12352202
• NACRES: NA.52

Propiedades

• grade: for molecular biology
• product line: BioReagent
• form: lyophilized powder
• impurities: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• suitability: suitable for nucleic acid hybridization
• storage temp.: 2-8°C

Descripción

General description

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Application

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

Components

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

Preparation Note

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)