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MilliporeSigma

14361C

SAFC

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

Animal-component free, with hypoxanthine, with thymidine, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fabricación farmacéutica

Sinónimos:

EX-CELL® CD CHO Serum-Free Medium for CHO Cells, Chemically Defined

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: 2.10 g/L
phenol red: no
HEPES: no
glucose: 5.6 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

EX-CELL CD CHO is a chemically defined, animal-component free, serum-free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells and expression of antibodies or protein products in suspension cell culture. CHO suspension cultures can be subcultured directly into EX-CELL CD CHO from serum-supplemented or serum-free medium with little or no adaptation. EX-CELL CD CHO (Catalog No. 14361C) is formulated with hypoxanthine and thymidine, making it an appropriate medium for applications that do not require the selective pressure of a hypoxanthine/thymidine (HT)-deficient medium, such as CHO-K1 and Glutamine Synthetase (GS System) users.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
GS System is a trademark of Lonza Group Ltd.

supplement

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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Artículos

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).

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