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14365C

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EX-CELL® CD CHO Fusion

Animal-component free, without L-glutamine, without hypoxanthine, without thymidine, liquid, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

sterility

sterile-filtered

product line

EX-CELL®

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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General description

EX-CELL® CD CHO Fusion is a chemically defined, animal-component free medium developed for the long-term growth of Chinese Hamster Ovary (CHO) cells. The absence of any large macro molecules allows for isolation and purification of secreted proteins from the cells. This medium is supplied without L-glutamine to aid in media stability, to avoid L-glutamine degradation that causes ammonia build-up and to provide an appropriate medium for the culture of CHO cells using glutamine synthetase selection (example: MilliporeSigma’s CHOZN GS knockout cell line). This medium does not contain hypoxanthine or thymidine to allow for its use with dihydrofolate reductase(DHFR-) gene amplification systems (example: MilliporeSigma’s CHOZN DHFR knockout cell line).

Quantity

Formulated to contain 20.9 grams of powder per liter of medium.

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
SLCJ3623
SLCL2847
SLCH7915
SLCJ9033
SLCH7176

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Qiong Wang et al.
Biotechnology and bioengineering, 116(9), 2303-2315 (2019-05-08)
With the increasing demand to provide more detailed quality attributes, more sophisticated glycan analysis tools are highly desirable for biopharmaceutical manufacturing. Here, we performed an intact glycopeptide analysis method to simultaneously analyze the site-specific N- and O-glycan profiles of the
Noriko Yamano-Adachi et al.
Scientific reports, 10(1), 17612-17612 (2020-10-21)
Chinese hamster (Cricetulus griseus) ovary-derived Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for the industrial production of recombinant therapeutics because of their ability to fold, assemble, and perform post-translational modifications, such as glycosylation, on proteins.
Weiyi Liu et al.
Biotechnology progress, 37(1), e3061-e3061 (2020-08-05)
Antibody-dependent cellular cytotoxicity (ADCC) is the primary mechanism of actions for several marketed therapeutic antibodies (mAbs) and for many more in clinical trials. The ADCC efficacy is highly dependent on the ability of therapeutic mAbs to recruit effector cells such

Artículos

Preparing CHO Cells for Higher Productivity by Optimizing a Perfused Seed Train

This application note describes the benefits and performance of Cellvento® 4CHO-X Expansion Medium designed for multiple cell expansion steps from vial thaw to N-1 stages to generate higher biomass for inoculating fed-batch production bioreactors

Cell Lines for Recombinant Protein Expression

Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines.

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