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Key Documents

43925

Sigma-Aldrich

Nigrosin Stain solution

for microscopy

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

Bacillus spp.
Corynebacterium spp.

General description

Negative staining method permits visualization of the usually transparent and unstainable capsule of many organisms, most importantly Cryptococcus neoformans.

Components

Composition:
Nigrosin 100 g/l, Formalin 5 ml/l in water

pictograms

Health hazardExclamation mark

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves


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A L Fedorovtsev
Sudebno-meditsinskaia ekspertiza, 35(2), 28-30 (1992-04-01)
The morphology of isolated cells of multi-layer squamous non-horny epithelium (buccal, vaginal, urethral, rectal), stained with 0.05% nigrosine solution, was under study, and the sizes of these cells and their nuclei were measured. Specific markers were detected in the buccal
A Novelli
Pathologica, 88(3), 188-191 (1996-06-01)
Nigrosin, an aqueous blue-black acid dye of the azine series, in conjunction with Biebrich scarlet, orange G and formic acid as mordant, it is found an excellent triple panchromatic rapid stain for histological purposes. The staining so obtained permits a
L Björndahl et al.
Human reproduction (Oxford, England), 18(4), 813-816 (2003-03-28)
The one-step eosin-nigrosin staining technique for assessment of sperm vitality was developed in the 1950s for various mammalian species. Although commonly used on human sperm in semen, a validation for this use has not previously been published. The technique was
Shuai Zhang et al.
Journal of colloid and interface science, 316(1), 168-174 (2007-08-31)
A convenient approach was developed to fabricate monodisperse nigrosine-doped poly(methyl methacrylate-co-divinylbenzene-co-methacrylic acid) nanoparticles with different cross-linkage by soap-free emulsion polymerization at boiling status and swelling process. The dye-doped nanoparticles were used for the fabrication of colloidal crystal films and beads.
D A Hewitt et al.
Animal reproduction science, 51(4), 321-332 (1998-08-01)
The processes of capacitation and acrosomal exocytosis of dog spermatozoa in vitro have yet to be fully investigated. Firstly, we investigated the effectiveness of a technique for staining dog spermatozoa with the fluorescent labels Hoechst 33258 and chlortetracycline. A modified

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